Dear Dr.Richard, Your paper gave a good example for us. Is it possible that you send me all the related files? It will be very helpful for students to learn the detail of solving difficult protein structures.
Thanks in advance! best, Zhonghao Chen China agricultural university. chenzhonghao...@163.com From: Tanner, John J. Date: 2017-07-18 00:34 To: CCP4BB Subject: Re: [ccp4bb] A challenging Molecular replacement Richard, I can’t help you with 5XQL. However, I can point out a recent structure from my group that might be useful for teaching. The structure was solved by MR with a search model that had 33% sequence identity and represented only 46% of the target structure. The Methods section of the paper has a detailed description of the phasing procedure. We used BALBES, then did autobuilding in phenix from the BALBES/REFMAC map. https://www.ncbi.nlm.nih.gov/pubmed/28420730 John J. Tanner Professor of Biochemistry and Chemistry Chair, Biochemistry Department Graduate Admissions Committee Department of Biochemistry University of Missouri-Columbia 117 Schweitzer Hall 503 S College Avenue Columbia, MO 65211 Phone: 573-884-1280 Fax: 573-882-5635 Email: tanne...@missouri.edu http://faculty.missouri.edu/~tannerjj/tannergroup/tanner.html Lab: Schlundt Annex rooms 3,6,9, 203B, 203C Office: Schlundt Annex 203A On Jul 17, 2017, at 11:01 AM, CDaddy <2295867...@qq.com> wrote: I am a structural biologist who is teaching X-ray crystallography. Recently I noticed that BrlR structure (5XQL) was solved using molecular replacement with a search model of very low similarity. I am very interested in this structure because I think this a very good example to show students how to solve phase problem using molecular replacement, especially when the model and the target protein share a low sequence identity. However, when I downloaded the data from PDB, I found that I cannot solve the phase problem using Phaser as mentioned by the authors. During this procedure BmrR (PDB:1R8E) was used as the search model. I tried to consult the authors for help but receive no response by now. Since the description of this issue in the literature is very brief, could anyone please spend a little time on this molecular replacement and give me some advices on this issue? I like to learn some valuable tricks. Your assistance will be highly appreciated. All the best, Richard.
