Hello, I am working on DSF to verify if some compounds bind to my protein. I see a negative shift of about 3-4 degrees upon ligand addition (dose-response) in comparison to the protein alone. I assume that this might be due to the binding of compound to the unfolded stated rather than folded protein.
In such a situation where compounds are to be screened with the aim of drug discovery, are these negative thermal shift compounds relevant and how can they be followed upon, or they should simply be discarded? Thank you.
