Ahh, this brings back memories of a former life, preparing hydrophobic coverslips for my surface chemistry experiments. I used chlorotrimethylsilane, and what I remember best is that the secret to a good coating is making sure your coverslips are utterly clean and dry. I used to clean them by boiling in base piranha (concentrated ammonia and hydrogen peroxide - need I say this stuff should be treated with extreme respect?) before rinsing thoroughly in milli-Q water and baking under vacuum. Then essentially as David said: dump them in the silane solution (again, it helps to make sure to keep your solvent dry), then fish them out one-by-one, rinse and dump them into a beaker of fresh solvent (acetone, I think I used). Then once they're all done, take them out of that, dry in a nitrogen stream and store. Painful & fiddly, but you can easily do a hundred or so in an afternoon.
Tristan Croll Research Fellow Cambridge Institute for Medical Research University of Cambridge CB2 0XY > On 27 Apr 2017, at 10:26, Praveen Kumar Tripathi > <tripathipraveen.i...@gmail.com> wrote: > > Dear all, > > sorry for off topic question. > > May i know if anybody uses homemade silinization of coverslips for protein > crystallization purposes? > > I have purchased Sigmacote SL2-100 ml for silanizing coverslips for hanging > drop protein crystallization setup. > > Please share your methods to siliconize coverslip using Sigmacote SL2-100 ml > if anybody uses. > > Thanks in advance > > regards > Praveen > > > > -- > Praveen Kumar Tripathi > PhD Research Scholar > Kusuma School of Biological Sciences > Indian Institute of Technology Delhi-110016 > +91-9873625228
