Hi all, I am solving structure of one of the acyltransferse protein. We have collected data at 2.16A resolution. Currently the Rfree is 0.2508 and Rwork is 0.2042.
*My query is regarding NCS difference.* Under validation tool of coot while looking for NCS differene, i can find some residues with red bar. *Can some one suggest me how may i minimize it if i am expected to do it?* -- Vipul Panchal Senior Research Fellow, Respiratory disease and biology, CSIR-IGIB (M)-9540113372