Hi Fellows,
a humble question for our glyco-expressionists: I have mutated out the Asns of the N-glycoslation consensus sites for Asp (Asp simply because the PNGaseF treated protein stays stable so I thought that might be a good guess) and indeed the unglycosilated mutant expresses well and gets secreted as planned. But rumor has it that glycoproteins that are mutated to non-glyc often are not processed correctly and that we had just dumb luck. May I poll the educated opinion of the erudite here? Cheers, BR ------------------------------------------------------ Bernhard Rupp Crystallographiae Vindicis Militum Ordo <http://www.hofkristallamt.org/> http://www.hofkristallamt.org/ <mailto:b...@hofkristallamt.org> b...@hofkristallamt.org +1 925 209 7429 +43 767 571 0536 ------------------------------------------------------ :(){ :|: & };: ------------------------------------------------------
