Dear Debiasish, it looks like you got spherulites. You might try to optimize the conditions or use these spherulites for microseeding. It worked for me once. Good luck!
Mirella Sent from my iPhone On 24 Mar 2017, at 08:29, Camillo Rosano <camillo.ros...@gmail.com<mailto:camillo.ros...@gmail.com>> wrote: Dear Debiasish, more than "quasicrystals" (crystal in which molecules adopt an ordered but aperiodic structure), to me the one in your photo looks like spherulites. You shouldn't be far for finding the right crystallization conditions: maybe change the pH of your precipitant and try lower concentration of protein or precipitant or both at the same time. Camillo On Fri, Mar 24, 2017 at 12:10 AM, Debasish Kumar Ghosh <dkgh...@cdfd.org.in<mailto:dkgh...@cdfd.org.in>> wrote: Dear All, I have a small doubt regarding possibility of formation of quasi-crystal of protein complex. I am trying to co-crystallize a heterodimeric protein complex of two small proteins (14KDa and 16KDa, both are human origin). One of them is a membrane protein. we have extensively characterized their qualitative and quantitative binding properties (with IP/IB, Confocal microscopy, ITC etc.). We are quite certain of their strong 1:1 binding stochiometry. In some crystallizing conditions, we are getting some structures which appears to me as quasi-crystals (Image attached). However, I am not coming across ample examples of quasi-crystals of protein complexes. I will be very keen to know if anyone had this (similar) kind of experience(s). And of course it would be wonderful to know if they are diffractable and, if yes, what are the odds of having good diffraction. Best !! Debasish CSIR- Senior Research Fellow Computational and Functional Genomics Group Centre for DNA Fingerprinting and Diagnostics Hyderabad, INDIA
