Ankita,
Fc are different from Fabs. Even if glycosylated their solubility is lower.
Fc stands for constant fragment but also for Fragment crystallizable:
https://en.wikipedia.org/wiki/Fragment_crystallizable_region
From its name, they should be easy to crystallize. If you have problems to
not hesitate to ask questions.
Enrico.
On Tue, 07 Mar 2017 07:14:34 +0100, Ankita Srivastava
<ankitas.stanf...@gmail.com> wrote:
Dear CCP4 members,
I am trying to crystallize an antigen-bound to the Fc fragment of an
antibody.
Is Fc fragment difficult to crystallize compared to Fab? Are there any
commercial screens widely known to crystallize Fc fragment or
antibodies?
I would appreciate if you can share any experience with crystallizing Fc
fragment or Fc-complex with other proteins.
Many thanks in advance!
Ankita
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
e-mail: est...@cea.fr Fax: 33 (0)1 69 08 90 71
Proxima-2A, Soleil Synchrotron. Tel: 33 (0)1 69 35 8180 Beamline
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