Hi all,
I have purified a protein, the gel filtration and Native-PAGE shows it is very homogeneous. There is no problem when I concentrated it to 20mg/mL. However, I found above 85% conditions will cause protein precipitation when I try to crystallize it even at 2mg/mL. I also tried to add 5% glycerol to the protein and there is no improvement. In addition, I found when dilute the well solution to 50% will not precipitate protein, but 70% or higher will cause. My protein is stocked in the buffer 20mM Hepes, pH 7.5, 150mM NaCl,5mM DTT and the PI is about 5.5.So, any suggestions are welcome? Thanks very much! Jiuwei
