Dear Madhurima, More information is needed regarding data processing/scaling, initial space group, NCS etc...Then the community can help you better. Anyway, I hope the crystal you got and collected the data are your target protein's...How sure you are you? How purified your sample is? Did you dissolve one crystal and ran a SDS gel... Of course if you have plenty of crystals and want to sacrifice one/two of them.
Good luck, Sudipta. On Feb 8, 2017 10:19 PM, "Madhurima Roy" <madhurima2...@gmail.com> wrote: > Hi all, > > I have a small protein which is of 6 kDa including six histidine tag. The > protein crystallized in the conditions given bellow : > > a) 0.1M Sodium acetate trihydrate pH 4.6, 3M NaCl. > b) 0.1 M Bis-tris pH 5.5, 2M Ammonium sulphate. > > The crystals are plate shaped in both conditions.We collected diffraction > data at our home source using Rigaku RaxisIV and processed the data using > XDS. > > In spite of good homology, the protein structure cannot be solved by > MolRep , the contrast is very low approx 1.65. The PDB Blast result is > given below: > > 38.1(total score), 70%(query coverage) 1e-05 (E-value) and 50%(Identity) . > > The screen shot of the statistics showing R factor and quality of fit in > CORRECT.LP file is enclosed below. > > > > Kindly help. > > > Thanks in advance. > > Madhurima > > > > > > >
