I solved a structure years ago (1L31 and a couple related entries) which had intermolecular oligomerizing disulfides in some of the crystals (reciprocal C23-C27’ and C23’-C27). I thought at the time that they seemed too “deliberate” to be just an artifact, but others thought that since the enzyme was intracellular, the disulfides would not be physiologically relevant. I still think they’re too perfect to be artefactual, and since the protein comes from Methanobacterium thermoautotrophicum, all usual bets might be off. I’ve always wondered about it, although I am not sure how profound the consequences are. I would love to hear peoples’ two cents on this.
Jacob From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Eleanor Dodson Sent: Wednesday, February 01, 2017 10:18 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] intermolecular dissulphides Does anyone know of examples of these? I have found one - 2WQW with these SSBOND records 2WQW SSBOND 1 CYS A 206 CYS A 227 1555 6556 2.07 SSBOND 2 CYS B 206 CYS B 227 1555 5556 2.15 We seem to have one but it would have to form after crystalisation? Eleanor
