Dear all, I wondered if it is OK to pipette ligand soln (X-CoA) *directly* to the drop with crystal (1:1 ratio of protein:precipitant 2µl) instead of dissolving it in precipitant solution and transferring the crystal to this ligand containing precipitant solution. The crystals survive this as I add the ligand solution to the edge of the drop and gently mix the two solutions. Since I collected my datasets I wonder if it is OK?
Many thanks, Markus
