Hello everyone, Please allow me to kill two off-topic birds with one email ‘stone’:
1) Can anyone suggest where I can lay hands on the pREP4-GroESL plasmid for bacterial co-expression of GroEL and GroES? I’ve checked with the original author (Philip Cole), but it’s been decades since he published on this, and he’s not sure he can find the plasmid. However, I see recurrent (and recent) references to the plasmid in the literature, so I guess that it’s loose in the wild… 2) We’re looking for a contract research organization to do some Bac-Mam expression for us (we’re in a hurry to get some results, so we’d like to farm it out rather than get the technology going in-house). Can anyone recommend a group (ideally in the US, for logistical ease)? Thanks for any help. Cheers, Pat --------------------------------------------------------------------------------------- Patrick J. Loll, Ph. D. Professor of Biochemistry & Molecular Biology Director, Biochemistry Graduate Program Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102-1192 USA (215) 762-7706 pjl...@gmail.com pl...@drexelmed.edu
