Excellent point. I've heard apocryphal stories of someone obtaining beautiful salt crystals (magnesium ammonium phosphate) by trying to crystallize an ATPase with ADP and Mg++ using (NH4)2SO4 as a precipitant. Wait long enough, and the ADP becomes ATP plus AMP, the ATPase takes the 3rd phosphate off, and then the salt crystals grow.
Yours sounds like a much less depressing "accident"! ________________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Phil Evans [p...@mrc-lmb.cam.ac.uk] Sent: Thursday, January 19, 2017 4:37 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15) Remember that 2xADP can disproportionate into ATP + AMP > On 19 Jan 2017, at 20:54, Panneerselvam, Saravanan > <saravanan.panneersel...@desy.de> wrote: > > Dear All, > Sorry for the little bit off topic. Is there a possibility for covalent > bond formation between beta phosphate of ADP and acetate molecule both > are coordinated by divalent metal ions? I am working on a Kinase > structure which was crystallized with ADP and in presence of 1M sodium > acetate. We observed additional density around ADP that fits perfectly > like a gamma phosphate , mimicking like ATP bound state, surrounded and > coordinated by two metal ions(resolution is 1.4A). There is a change in > space group (from I212121 to P212121 ) and further important > conformation changes are observed around ATP binding pocket and distant > region. This is the only xtal we obtained in this space group, and all > other xtals(measured 10 xtals) from the same plate belong to I212121. > > > > Thanks your help and time! > > Saravanan > ----- Original Message ----- > From: "CCP4BB automatic digest system" <lists...@jiscmail.ac.uk> > To: CCP4BB@JISCMAIL.AC.UK > Sent: Sunday, 15 January, 2017 01:01:37 > Subject: CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15) > > There are 2 messages totaling 45 lines in this issue. > > Topics of the day: > > 1. Phenix (2) > > ---------------------------------------------------------------------- > > Date: Sat, 14 Jan 2017 20:58:09 +0000 > From: D Bonsor <dbon...@ihv.umaryland.edu> > Subject: Phenix > > Is the phenix website down? Anyone know when it will be back up? > > ------------------------------ > > Date: Sat, 14 Jan 2017 14:45:06 -0800 > From: Pavel Afonine <pafon...@gmail.com> > Subject: Re: Phenix > > See notice on Phenix mailing list that answers your question. > > On Sat, Jan 14, 2017 at 12:58 PM, D Bonsor <dbon...@ihv.umaryland.edu> > wrote: > >> Is the phenix website down? Anyone know when it will be back up? >> > > ------------------------------ > > End of CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15) > ************************************************************
