Dear Alex,
Refmac doesn't do density modification. If you want take advantage of
better starting phases you have to do DM prior Refmac or model building
with Buccaneer for example. I recommend PARROT for that.
I would use i2 Parrot and this as input in the Buccaneer pipeline with
precomputed phases form Parrot.
Cheers
Christian
Am 16.12.2016 um 19:44 schrieb Alex Lee:
Hi All,
I am using CCP4i 7.0.0.025 linux version. I am wondering if Refmac5
automatically does density modification (e.g. solvent flattening, NCS,
etc..) in the refinement after I put a solution coordinate from Phaser
MR molecular replacement and the experiment data (MTZ file)? If
Refmac5 does not do density modification automatically, is it better
that first doing a density modification (CCP4i DM or Pirate) after
molecular replacement (input the solution coordinate file and mtz map
generated by Phaser MR), and then building structures and finally do
the Refmac5?
