In this case, calculating Matthews coefficients / solvent fractions for different possible complexes will be helpful.
http://www.ccp4.ac.uk/html/matthews_coef.html In the general case, without solving the structure I would dissolve the crystals and run them on an SDS-PAGE gel, then silver stain to visualize both RNA and protein. Best wishes Kevin On Thu, Dec 15, 2016 at 9:53 AM, Liu Rachel <liuyujie1...@hotmail.com> wrote: > > Dear everyone: > > > Recently, I suffered a problem during my research work. I co- > purified a zinc finger protein(152aa) and a dsRNA(19bp), the final SEC > buffer is *20mM Hepes, 150mM NaCl, pH8.0*. Then the complex was > co-crystallized > by vapor diffusion against a solution of *30% **PEG400, 0.2M MgCl, 0.1M > Tris *pH8.4. The crystal is very beautiful, but the X-ray diffraction > diagram is > very strange, the diffraction point looks big and sparse(as shown in the > picture). The Data cannot be processed with HKL2000 either. > > unit_cell = 24.808 42.863 119.042 90 90 90 > > space_group = "I 2 2 2" > > > > I wanna figure out, could this be a complex crystal? or only RNA > crystal? or other micromolecular? > > > > PS. I've set drops without the protein in the sample, but prepare the > sample of RNA with the protein buffer as if the protein was there. And > there was no crystallization . > > > Thank you very much! > > > > > > Rachel Liu > > Room 2071, research center in life sciences, > > China Agricultural University > > No. 2 yuanmingyuan west road, Haidian District, Beijing, 100193 P.R. China > > Tel: (86)-10-62734078 > >
