Hi Harry, Thank you very much for the information! I am also looking forward to the online tutorial!
On Sun, Dec 4, 2016 at 4:55 AM, Harry Powell <ha...@mrc-lmb.cam.ac.uk> wrote: > Hi Alex > > (1) Go to “Settings -> Experiment settings -> Detector” and change the > value there; you should probably re-run integration, though since your > value is only just outside the range of 0.7 - 1.3, I might not worry too > much about that, and I don’t think it would make a big difference. > > (2) Don’t worry about this too much if it only happens for two images. > Have a look at the offending images, and if > > (a) there is anything _obviously_ different about them compared to the > preceding and following images, you _could_ omit them from the scaling (I > wouldn’t bother to re-run the integration). > > (b) they look broadly similar to the preceding and following images, I > wouldn’t worry at all. > > (c) if this happened for a large proportion of the images I would be more > concerned (which leaves the question, “what is a large proportion”?). > > (3) My view is that most of the “warnings” in Mosflm are advice that can > be used to improve data processing; you will learn which are more important > through the lens of experience. > > (4) I’d also work through the online tutorial which will give you a pretty > well comprehensive introduction to everything there is to know about > changing parameters etc in iMosflm. It takes anywhere from 1 to 4 hours to > work through, depending on how carefully you do it. > > HTH > > Harry > -- > Dr Harry Powell > Chairman of International Union of Crystallography Commission > on Crystallographic Computing > Chairman of European Crystallographic Association SIG9 > (Crystallographic Computing) > > On 3 Dec 2016, at 21:48, Alex Lee <alexlee198...@gmail.com> wrote: > > Dear CCP4bb members, > > I am new to iMosflm data processing (I use HKL2000 but I really want to > learn iMosflm). After automatic index, cell refinement and integration in > iMosflm, I got 2 warnings. > > 1. Error in detector gain. BGRATIO of my data lies outside the 0.7 to 1.3 > range (mine is 0.64) Imosflm suggests changing GAIN to 0.14. How do I > change GAIN to the 0.14 value? Do I need to change GAIN before integration? > > 2. Poor standard profiles in some areas. Both Image 1 and Image 13 have > some boxes with cc lower than 0.5. Should I exclude these two frames? How > to do it? I know in HKL2000 after scaling there is "exclude frames" > option, but I could not find it in iMosflm. > > Thanks. > > > >
