Hi Eike, I wouldn't necessarily let the morphology deter you. While working on my PhD, a colleague collected beautiful data from hollow crystals.
If the crystals collapse while looping, is it possible for you to perform "micro-surgery" on the crystal so that you only have a single face? Sometimes you can obtain very nice datasets from plates. Best, Chris On Wed, Nov 30, 2016 at 8:20 AM, Schulz, Eike-Christian < eike.sch...@mpsd.mpg.de> wrote: > Dear all, > > > > Is there a documented case of a structure determination from a hollow > crystal? We have the unfortunate situation that a protein only crystallizes > as a hollow tube, where the inside is filled with solvent, which ruins the > diffraction patterns. > > > > I was often confronted with similar situations, but in the previous cases > I could always find a suitable crystal (part) or a different > crystallization condition saved me. > > > > If anybody had general advise on how to proceed or could point me to > relevant literature it would be much appreciated. > > > > With best regards > > > > Eike > > > >
