Yes, it is many years ago, a large protein batch (50 ml or so) stored in
the cold room that produced an impressive layer of crystals at the
bottom of the flask.
One student at that time tested many crystals and found only a few that
diffracted to atomic (!) resolution.....
Crystallization is a usefull methodology for protein purification! What
I would do is to harvest these crystals (often more pure protein than in
the stock solution), dissolve them and try to grow new crystals as
proposed by Artem.
Good luck!
Jeroen
Am 18.10.16 um 12:41 schrieb kiki:
Dear all users,
I got my crystals at the bottom of the tube on ice just before I
started to screen crystals. Those crystals' shapes were good but easy
to melt when I harvested them. I shot these crystals. They only
diffracted to 7A to 8A. Has anyone ever met this situation before? How
to improve?
Thanks,
Lingyuan
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