Dear Andre, It is a small protein then, I recommend to do the purification in a small micelle detergent (e.g. OG). This regardless the extraction done in DDM or DM or any other suitable detergent.
You can start by reading Moraes, Isabel, et al. "Membrane protein structure determination—the next generation." Biochimica et Biophysica Acta (BBA)-Biomembranes 1838.1 (2014): 78-87 (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898769/) or Moraes, Isabel & Margarida Archer. "Methods for the Successful Crystallization of Membrane Proteins." Structural Proteomics: High-Throughput Methods (2015): 211-230 Best regards, Isabel -------------------------------------------------------------------- Dr Isabel Moraes Head of the Membrane Protein Laboratory Diamond Light Source Ltd, Harwell Science and Innovation Campus, Oxfordshire, OX11 ODE, UK -------------------------------------------------------------------- On 28 Jul 2016, at 12:47, Andre Godoy <00000b9d7671a1b6-dmarc-requ...@jiscmail.ac.uk<mailto:00000b9d7671a1b6-dmarc-requ...@jiscmail.ac.uk>> wrote: Dear community we are starting the study of a 20 kDa alpha transmembrane domain, and we were wondering if one could recommend any particular detergent (and its concentration) that won't influence the concentration step for crystallization. Tips for crystallization using detergents and LCP would also be helpful. any comment would be appreciated All the best, Andre Godoy -------------------------------------------------------------------- Dr Isabel Moraes Head of the Membrane Protein Laboratory Diamond Light Source Ltd, Harwell Science and Innovation Campus, Oxfordshire, OX11 ODE, UK http://www.diamond.ac.uk/Beamlines/Mx/MPL.html -------------------------------------------------------------------- -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
