Dear all, I want to get a complex of DNA-RNA-protein. But I have a big problem of annealing DNA-RNA. The length of DNA is 19nt and RNA is 17nt. Annealing protocol: 2uM DNA 2uM RNA 10mM Tris-Hcl 100mMNaCl 1mMEDTA
Heated to 95 for 5min, cooling down slowly for nearly 2h to room temperature. I can just get a result of two single strand DNA/RNA. PAGE analysis. No double helix was founded. Does anyone have the same problem or know how to fix it. Thank you for your answering. Best regards, Weifei
