Hi Mohan, I have seen these too with the C row of morpheus, unfortunately they are most likely to be salt. Try repeating the same screen but skipping adding extra Mg to the macromolecule.
Good luck. Regards Saba Shahzad | PhD candidate, Martin Hallberg group Department of Cell and Molecular Biology Karolinska Institutet ________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of jai mohan [jaimoha...@yahoo.co.in] Sent: Monday, May 18, 2015 8:25 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Crystal morphology Dear Bishwa, What about Hamptons Izit dye. S.M.Jaimohan, Ph.D On Monday, 18 May 2015 7:50 AM, Engin Özkan <eoz...@uchicago.edu> wrote: Magnesium phosphate crystals? I've definitely seen salt crystals with that morphology before. Check out the Ksp for Mg3(PO4)2. Engin On 5/17/15 6:45 PM, Bishwa Subedi wrote: > Hi All, > > I know that its hard to say if the crystal is protein crystal just by looking > at its morphology, however I was wondering if any of you have ever come > across protein crystals as one attached here. It would help build up my > confidence :) and work further until I put them on to X-ray beam. I could > notice similar crystals with two different proteins one in the initial screen > and one after pH screening the similar condition and involves NPS (Nitrate, > phosphate and Sulphate) from Morpheus screen. To mention, MgCl2 (0.01M) is > also added directly to the protein as a co-factor. > > Crystal condition : NPS (0.9M)+ Buffer System 2 0.1M (i.e. HEPES and MOPS) > as well as with buffer system 3 0.1M (TRIS and BICINE) at pH 7.5 and 8.5 > respectively and P550MME_P20K 30.0% > > Thank you for your opinion. > > Bishwa
