If in doubt, try dragging the crystal through a 1:1 mix of Paratone-N and Mineral oil until most or all the mother liquor from surrounding the crystal has been removed.
There are more tips here: http://hamptonresearch.com/tip_detail.aspx?id=99 Good luck! D On Mon, 4 May 2015 19:45 Faisal Tarique <faisaltari...@gmail.com> wrote: > Hello everyone > > Can anybody suggest me a cryo condition for a crystal obtained in > MIDAS screen of Molecular Dimension: > > G1> 0.1MTris8.0G10.1Mpotassium chloride25% v/vSOKALAN®CP7 0.1MHEPES7.0 > > G2>0.3Mammonium formate20% v/vSOKALAN® CP 5 0.1MHEPES7.0 > > Crystals are in beautiful cuboid shaped but all sorts of PEG > combinations and Glycerol formulation failed to prevent it from > cracking and dissolving. > > Has anybody faced a similar situation as mentioned above and what > precaution was taken to prevent it from cracking or dissolving. > > Your suggestions will be of immense help > > Thanks in advance > -- > Regards > > Faisal > School of Life Sciences > JNU >
