Sometimes (sadly, not always) the problem with thin plates is that they get damaged in mounting. If none of the tricks suggested work, you might try seeing if you get any better data from the existing crystals using those tennis racket shaped loops that give more support to the thin plate.
> On Apr 26, 2015, at 7:58 PM, Prerana G. <tracy...@gmail.com> wrote: > > Thanks a lot for your suggestions. > > Regards, > Prerana > >> On Fri, Apr 24, 2015 at 7:05 PM, Tanner, John J. <tanne...@missouri.edu> >> wrote: >> Following up on Dave's suggestion, you could try using crystal screens as >> additives. This has worked well in my lab. The idea is to mix the condition >> that you currently have (the "base") with all the crystal screen reagents >> you have in stock (CS, Index, etc.). As a first trial, use reservoirs >> containing 3 parts base and 1 part screen. This generates a new matrix of >> hits. You might find a condition that produces thicker crystals or perhaps >> a new new crystal form. I think this approach is described in the literature >> but don't remember the citation. >> >> Jack >> >> >> >>> On Apr 24, 2015, at 12:31 AM, David Briggs wrote: >>> >>> Hi, >>> >>> In my experience, additive screens (e.g Hampton's) can change crystal >>> morphology. You could also re-screen for new conditions either using matrix >>> micro seeding, or change the protein buffer. Perhaps adding a ligand or a >>> component from your current crystallisation conditions to your protein >>> stock? >>> >>> HTH, >>> >>> Dave >>> >>>> On Fri, 24 Apr 2015 04:02 Prerana G. <tracy...@gmail.com> wrote: >>>> Dear all, >>>> >>>> I am working on a protein (40kDa) which forms very thin plate shaped >>>> crystals which diffracts at very low resolution. Protein concentration >>>> that i have used for crystallisation is approx. 8mg/ml. I have attached >>>> the picture of the protein crystal. >>>> >>>> >>>> How can I improve upon the shape of the crystal? >> >> >> >> John J. Tanner, PhD >> Professor of Biochemistry and Director of Graduate Admissions and Recruitment >> Professor of Chemistry (Joint Appointment) >> University of Missouri-Columbia >> 125 Chemistry Building >> Columbia, MO 65211 >> email: tanne...@missouri.edu >> phone: 573-884-1280 >> fax: 573-882-2754 >> http://faculty.missouri.edu/~tannerjj/tannergroup/tanner.html >
