Dear All, Caused by poor resolution, I can only build a series of Ala into my helix density (for sure the density is for helix). Then I tried to use the dock sequence in the extension of the Coot to change to my target sequence. Coot says it was not confidence on the alignment, thus it did not change the Alas into my target sequence. In the "Molecule to be sequenced" in the coot dock sequence, if we paste the sequence, show we still need to input the pir file? For the sequenc we paste, it should be my target sequence rather than the series of Alas , right? Why in this graphical interface there are "Sequence closest fragment" and "Sequnece all fragments!"? I ask these questions in order to avoid my failling to do something which Coot can do. I am looking forward to getting your reply. Smith
-------- Forwarding messages -------- From: "Smith Liu" <smith_liu...@163.com> Date: 2015-04-01 12:58:01 To: lists...@jiscmail.ac.uk,"CCP4BB@JISCMAIL.AC.UK" <ccp4bb@jiscmail.ac.uk> Subject: on building a helix fragment by coot Dear All, If I need to build a fragment of helix to a fragment of density by coot, should I use baton build method or should I use place Helix here function? I have tried both, but I find it is difficult to place the sidechains to the map mosition which looks like where shold be occupied by the sidechains. I am looking forward to getting your message on how to correctly buld the helix. Best regards. Smith
