There are 8 possible space groups in the P422 family. Did you search in all of these? How many search models are likely to fit in the unit cell based on Matthews analysis? Then there are questions about model selection and preparation. How identical is the search model to the target? Does it make sense to search for the domains separately? Did you truncate the model side chains?
There are lots of ways to skin the cat depending on the problems encountered. 3.0 A may be challenging for a low identity search model. __________________ Roger Rowlett Gordon & Dorothy Kline Professor Department of Chemistry Colgate University On Apr 1, 2015 11:06 AM, "Sekharreddy M" <vijayasekk...@gmail.com> wrote: > HI All, > I am trying to find MR solution for one of the protein crystals > with 3.0A resolution, but not able find solution using Phaser (PHENIX).The > protein has two domains.and the probable best space group identified was > p4212(HKL2000). > > I am novice in using crystallization tools for solving structures.I am > stuck at this stage and I appreciate any help in this regard. > > Thank you. >
