Hi Reza. Clearly nobody needs to know anything about what protein you are specifically working on; that being said, in order to avoid a potentially endless email string of expert advices, please include everything detail-wise regarding your expression system, culture conditions, induction, and lysis method for BOTH the 3 ml and 500 ml expression trials. You will get, I imagine, amazing advices likely specific enough to solve the problem without your having to chase your tail. Best, John Fisher
John C. Fisher, M.D./PhD On Thu, Mar 19, 2015 at 2:33 PM, Reza Khayat <[email protected]> wrote: > Hi, > > > > We can express quite a bit of soluble protein when growing 3ml cultures. > However, the protein becomes insoluble (inclusion bodies) when we scale up > to 500ml cultures. Has anyone experienced such a problem, and found a > solution to it? Thanks. > > > > Best wishes, > > Reza > > > > Reza Khayat, PhD > > Assistant Professor > > Department of Chemistry > > City College of New York > > New York, NY 10031 > > http://www.khayatlab.org/ > > 212-650-6070 > > >
