Do you have evidence that the oil blocks diffusion of O2? O2 is a nonpolar
molecule, generally much more soluble in oils than in water. I'm not sure about
silicone oils, but I would think they also dissolve O2 readily.
eab
On 03/18/2015 08:02 AM, Patrick Shaw Stewart wrote:
Hi Steve
I have one more comment for this thread.
The microbatch-under-oil method is very handy for anaerobic work:
1. You can keep the microbatch stock solutions in normal microtitre plates
(polypropylene is best to reduce evaporation) for months, which hugely reduces
the amount of degassing that you need to do. You will only use say 0.5 ul of
stock per drop.
2. The oil offers a surprising amount of protection from oxidation, which
may be helpful eg in harvesting.
3. Microbatch can be automated - in parallel to vapor diffusion if desired
It's amazing how often (aerobic) microbatch produces far superior crystals to
V.D. for no obvious reason - it's well worth trying for both screening and
optimization.
Best wishes
Patrick
On 11 March 2015 at 10:17, <Stephen Carr> <stephen.c...@rc-harwell.ac.uk
<mailto:stephen.c...@rc-harwell.ac.uk>> wrote:
Dear CCP4BBer's
Apologies for the off-topic post, but the CCP4BB seems to be the best place
to ask about crystallisation.
I am looking to set up crystallisation in an anaerobic glove box and
wondered how other people did this, specifically the crystallisation stage. My
initial thoughts were to place a small crystallisation incubator inside the
box, however the smallest I have come across so far (~27L) is still rather
large. Has anyone come across smaller incubators? Alternatively are
incubators even neccessary if the glove box is placed in a room with good air
conditioning and stable temperature control?
Any recommendations would be very helpful.
Thanks in advance,
Steve Carr
Dr Stephen Carr
Research Complex at Harwell (RCaH)
Rutherford Appleton Laboratory
Harwell Oxford
Didcot
Oxon OX11 0FA
United Kingdom
Email stephen.c...@rc-harwell.ac.uk <mailto:stephen.c...@rc-harwell.ac.uk>
tel 01235 567717 <tel:01235%20567717>
This email and any attachments may contain confidential, copyright and or
privileged material, and are for the use of the intended addressee only. If you
are not the intended addressee or an authorized recipient of the addressee,
please notify us of receipt by returning the e-mail and do not use, copy,
retain, distribute or disclose the information in or attached to this email.
Any views or opinions presented are solely those of the author and do not
necessarily represent those of the Research Complex at Harwell.
There is no guarantee that this email or any attachments are free from
viruses and we cannot accept liability for any damage which you may sustain as
a result of software viruses which may be transmitted in or with the message.
We use an electronic filing system. Please send electronic versions of
documents, unless paper is specifically requested.
This email may have a protective marking, for an explanation, please see:
http://www.mrc.ac.uk/About/informationandstandards/documentmarking/index.htm.
--
patr...@douglas.co.uk <mailto:patr...@douglas.co.uk> Douglas Instruments Ltd.
Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK
Directors: Peter Baldock, Patrick Shaw Stewart
http://www.douglas.co.uk
Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034
Regd. England 2177994, VAT Reg. GB 480 7371 36
