On Tuesday, 17 March, 2015 17:34:24 Manish Shah wrote: > Hi All, > > I solved a structure using molecular replacement (Phaser) and the current > R-factor and R-free is 0.2 and 0.25. The electron density looks great for > residues and the ligand, however, the issue is with the B-values. The > average B-value is 110 at this stage of refinement and I could not bring it > down. All the residues are having a high B-values ranging from 90-120, > while the R is good. I have tried several options in CCP4 (restrained > refinement in Refmac) to bring it down and also used other MR programs, but > still it still continues to be in the same range. > > I will look forward to any suggestions. Thank you.
What was the Wilson B factor reported by Aimless or Truncate? What is the resolution of the data? -- Ethan A Merritt Biomolecular Structure Center, K-428 Health Sciences Bldg MS 357742, University of Washington, Seattle 98195-7742
