> I use a syringe with a needle and poke through the tape into the reservoir and top it off
Another approach is to poke holes in the tape with a needle, and just let the drops slowly evaporate and dry out. I'm told it works! On 17 February 2015 at 09:19, Bernhard Rupp <hofkristall...@gmail.com> wrote: > Just a possibility for salvage of your already set-up drops: > > > > You can spike the reservoirs with some highly concentrated precipitant (no > matter what as long as > > it sucks more water out of your drop). It does not solve your problem but > maybe you can > > revive a few drops and get more information from your experiment. > > I use a syringe with a needle and poke through the tape into the reservoir > and top it off with the > > high conc. precip. The tiny hole is easy to re-tape and does not hinder > observation. > > > > Best, BR > > > > *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of * > Mattiroli,Francesca > *Sent:* Tuesday, February 17, 2015 5:23 AM > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] how to reduce protein solubility > > > > Hi all, > > I am struggling with a protein complex that is too soluble. I have reached > about 20 mg/ml but I still observe very little precipitation (clear drops > in 90-95% of the tested conditions). The proteins are expressed in insect > cells and going to higher concentration is not easily achievable. > I have tried different buffer conditions (salt concentration and pH) and I > am testing temperatures. I am at a loss with what to try next. > Do you think PTMs (phosphorylation, acetylation) might be causing this? > Any input on how to decrease solubility? > > Thank you very much in advance, > > Francesca > > -- patr...@douglas.co.uk Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36
