Hi Robbie, thanks a lot. I was suspecting that editing the PDB was probably the easiest way to go. I will try that!
Best, Almudena 2015-01-26 10:44 GMT+01:00 Robbie P. Joosten <r.joos...@nki.nl>: > Hi Almudena, > > You can use Cootilus/Nautilus to rebuild the nucleic acid in your current > density. This is not a direct conversion, but it should be quick enough. > The conversion form RNA to DNA can be done (outside COOT) in a text editor > by changing the residue names an deleting the O2' atoms. If you rename > uridine (U) to deoxyuridine (DU), you can use 'simple mutate' in COOT to > convert it into thymidine (DT). > The conversion DNA to RNA is more complicated because you need to add the > O2' atoms. Unfortunately, (in my hands) the fill partial residues function > in COOT does not add the missing O2' atoms. You can delete and re-add the > residues, but that is really slow. > > HTH, > Robbie > > > On 01/26/2015 10:01 AM, Almudena Ponce Salvatierra wrote: > >> Dear all, >> >> is there a "fast" way of changing the chain type from RNA to DNA or from >> DNA to RNA within coot? I have already quite a bit built into density, >> but it is the wrong kind of nucleic acid. >> >> Best, >> >> Almudena >> >> -- >> Almudena Ponce-Salvatierra >> Macromolecular crystallography and Nucleic acid chemistry >> Max Planck Institute for Biophysical Chemistry >> Am Fassberg 11 37077 Göttingen >> Germany >> >> -- Almudena Ponce-Salvatierra Macromolecular crystallography and Nucleic acid chemistry Max Planck Institute for Biophysical Chemistry Am Fassberg 11 37077 Göttingen Germany
