Karplus and Diederichs. Science 2012. Hendrickson. Science. 2012
some references :-) 2015-01-16 16:01 GMT+01:00 Almudena Ponce Salvatierra <maps.fa...@gmail.com> : > Dear Izuok, > > 1. There are different metrics you can look at in order to know on how > much anomalous signal you can rely: CCano, ΔFano/σΔF, and ΔFano/F, In the > CCP4 study weekend in Nottingham last weekend, Prof. Janet Smith said that > for them it was the last one, ΔFano/F, the one working better as an > overall estimate of the anomalous signal. Otherwise, you can look at the > XDS output and see where the CCano drops below 0.3, or where the SigAno > drops below 1... that should give you an idea. Otherwise put your mtz > through Phenix Xtriage, it will output a good analysis of the data as well > as reccommend a resolution cutoff for anomalous resolution. > > 2. I don't really know... well if you are working with a protein, there > might be sites (Cys residues) where Hg would be more likely to be found... > But I am not sure here... I would use as a first guess same number of sites > as of Cys residues... but I am not working with proteins so I'm just > guessing... probably there is a better way. > > All the best, > > Almudena > > 2015-01-16 15:33 GMT+01:00 luzuok <luzuo...@126.com>: > >> >> Dear all, >> I'm doing Hg SAD phasing for the first time, and I met some problem: >> 1. How to assess the anomalous signal after I process the data and get my >> mtz file? >> My labmate doesn't scan the fluorescence signal. Actually, I don't >> quite understand why we use fluorescence to detect anomalous signal. >> 2. How to estimate the number of heave atom in one unit cell? by soaking >> heavy atom derivatives. >> >> Best! >> >> Lu Zuokun >> Nankai University >> >> -- >> 卢作焜 >> 南开大学新生物站A202 >> >> >> > > > -- > Almudena Ponce-Salvatierra > Macromolecular crystallography and Nucleic acid chemistry > Max Planck Institute for Biophysical Chemistry > Am Fassberg 11 37077 Göttingen > Germany > > -- Almudena Ponce-Salvatierra Macromolecular crystallography and Nucleic acid chemistry Max Planck Institute for Biophysical Chemistry Am Fassberg 11 37077 Göttingen Germany
