-----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Dom Bellini Sent: Thursday, January 15, 2015 12:25 PM To: [email protected] Subject: Re: [ccp4bb] X-ray Source Differences (WAS: RE: [ccp4bb] How far does rad dam travel?)
Dear Jacob, To add one more thing to the many ones from James, despite air scattering noise being flat and easily subtractable over time, that noise wont be the same if you take images with and without the sample. Since some of the photons will be absorbed and diffracted by the crystal, the air noise will be different from what you recorded without the sample (with or without the loop/stuff) and you would like to subtract. Right--I meant to extrapolate the background from non-spot pixels on the detector. I am still processing James's message, however (always a good idea to consider them well). BTW, I should add that many of these statistical discussions on CCP4BB have informed a lot of (all?) other research I have been doing, so thanks very much everyone for the enlightenment! JPK
