Dear Dialing, Just in case, make sure you get the residue numbering right. I.e., if you are try to insert a new residues between two residues already consecutively numbered, for example 96-97 or 134-135, then Coot will not to create the connection with the new residue.
HTH D -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Tim Gruene Sent: 14 January 2015 14:48 To: ccp4bb Subject: Re: [ccp4bb] Coot: How to connect N-terminal to neighbouring C-terminal -----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Dear Dialing, in that situation you only see the residues unconnected because they are too far apart. The refinement suggested by Zhijie Li would bring them in proper distance. Best, Tim On 01/14/2015 03:24 PM, Dialing Pretty wrote: > Dear All, Suppose I delete a residue ( residue 100 for example) for > outlier refinement, then I add the same residue at the C-terminal of > residue 99 (by Add terminal residue function of the Coot). By coot, > how can I connect the C-terminal of residue 100 to the N-terminal of > residue 101? I am looking forward to getting your reply. DIaling > - -- - -- Dr Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.4.12 (GNU/Linux) iD8DBQFUtoGQUxlJ7aRr7hoRAq2mAJ92A2SiNxBo3z42j5VEk4Zv5cTnUwCfQHeU cEJ4X31gzgDtZEKf6EcOnQ0= =+k9h -----END PGP SIGNATURE----- -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom
