Dear All I am running ITC experiment for my protein. At 250 nm Ligand concentration (protein was 40nm) I was getting exothermic thermogram. But it was not saturated. I reduced Ligand concentration to 15 nm and checked again. I got endothermic thermogram. I dont understand why I am getting both endothermic and exothermic thermogram for same protein and ligand. Can some one explain
Thank you Sajid
