Dear all, i am solving a structure of 2.5 A resolution by PHASER but every time i got this following warning.
$TEXT:Warning: $$ Baubles Markup $$ ------------------------------------------------------------------------------------- Large non-origin Patterson peak indicates that translational NCS is present: correction factors applied ------------------------------------------------------------------------------------- $$ $TEXT:Warning: $$ Baubles Markup $$ ------------------------------------------------------------------------------------- Solutions with Z-scores greater than 49.2 (the threshold indicating a definite solution) were rejected for failing packing test #1 TFZ=65.6 PAK=42 #2 TFZ=65.5 PAK=68 ------------------------------------------------------------------------------------- however every time PHASER run successfully and it give a output PDB as a solution. is it a right solution or not? or To get the right solution what should i do? if i used accept all the solution in expert parameter, i also got the solution but which does not follow the Packing test. Can anyone suggest... -- WITH REGARDS Rohit Kumar Singh Lab. no. 430, P.I. Dr. S. Gourinath, School of Life Sciences, Jawaharlal Nehru University New Delhi -110067
