Thank all of you for your inspiring information! Now I understand that actually axis order permutation and phase flipping can have the same effect on the map.
All the best, Steven On Wed, Oct 15, 2014 at 4:48 AM, Ian Tickle <ianj...@gmail.com> wrote: > The signs of the phases will depend on the permutation order of the axes. > Maybe when you created the map you inadvertently reversed the hand? What > do you see if you run mapdump on the map? > > Cheers > > -- Ian > > On 15 October 2014 01:53, Steven Chou <stevezc...@gmail.com> wrote: > >> Dear All, >> >> I used 'sftools (in CCP4)' to convert a map (in CCP4 format) to structure >> factors (.mtz). >> However the phases were flipped. I back converted it into a map file and >> compared it with the original one. The back converted one was just the >> mirror of the original one. To get the right phase, I had to convert the >> structure factors to a text format (CIF or XPLOR) and edit it with script. >> Their relationship is: CorrectPhase = 360 - phaseFromSftools. >> >> I also used CNS to do the same conversion. The phases from CNS were >> correct. >> >> sftools >> > set spacegroup >> > P1 >> > set cell >> > 200 200 200 90.000 90.000 90.000 >> > mapin input.map MAP >> > map2sf resol 3 column F_prot PHI_prot >> > write output.mtz >> > exit >> >> Was sftoold designed in this way? >> >> Thanks! >> Steven >> >> >> -- >> Steven Chou >> >> >> > -- Steven Chou
