Dear Bulletin Board, I am struggling with a protein domain of 15 kDa, with about 22 kDa carbohydrate attached. So far, the domain did not crystallize and I suspect the carbohydrate may hinder crystallization. Completely removing the carbohydrate results in low expression yields and poorly soluble protein, so I would like to try to remove some, but not all carbohydrate. Does anyone has a good protocol to trim, but not completely remove the carbohydrate?
Thank you, Herman Schreuder
