Dear All,

I have collected a diffraction dataset from a crystal soaked in a solution 
containing the ligand of interest.
After refining a few cycles, I can see some density in the active site pocket, 
but not so clear to model the ligand unambiguously.

Is any tool available to validate whether the ligand is actually there or not ?
The Twilight server appears to be for PDB files that have already been 
deposited.

thanks and regards,
Ansuman Biswas,
dept. of Physics,
Indian Institute of science

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