Hi,
I am working on a protein with a resolution of 2.8 A, I have scaled it and got solution of R-work and R-free of 0.38 and 0.43 by using 33% identical protein as a model. My protein exits as a dimer and crystallized the dimer. In solution I got only one molecule, i could see 80 % of sequence is matching with electron density also Mathew's coefficient says it is two molecules. How can I kept the previously modeled one and search for other molecule by using the refined mtz and pdb files?. Are there any programs in ccp4suite. please suggest me. -- ravi kumar
