Hi Rohit, Mass spectrometry might give you the most definitive answer, if you can denature or digest and identify the adduct.
In addition, PLP has a characteristic absorbance that can change based on its chemistry, and so you may be able to probe it spectrophotometrically, although this might not be as straightforward as MS Cheers, Shane Caldwell McGill University On Tue, Aug 19, 2014 at 2:56 AM, rohit kumar <rohit...@gmail.com> wrote: > Dear All, > i have solved a structure of 3.2 A. That is a PLP depended enzyme. > In their resting state, PLP-dependent enzymes are usually joined by a > covalent aldimine linkage to an essential lysine residue with a C=N bond. > This generates the so-called internal aldimine moiety. > Could anybody tell me how we can say that this PLP is covalently attached > to the Lys or its making Schiff base or not. > > -- > WITH REGARDS > Rohit Kumar Singh > Lab. no. 430, > P.I. Dr. S. Gourinath, > School of Life Sciences, > Jawaharlal Nehru University > New Delhi -110067 >
