Hello CCP4bb I am solving a 3.2A resolution crystal data for a protein DNA complex. I would like to know- 1) How to validate the DNA constraints in the crystal structure? 2) How to fit DNA into density? How different it is from protein fitting into density? 3) What kind of parameters to consider for refining DNA in Refmac for a Protein-DNA complex? 4) Which versions of refmac and COOT support DNA refining and model building?
Thank you in advance, Sasha Pausch
