Dear Atul,
I suggest to run SDS-PAGE with your crystals. You can even use
micro-crystalline precipitate that you probably have from optimization
stage. May be protein dissociated into subunits in the crystallization
conditions. But I am not sure because I know nothing about your protein.
Also, how do you cryoprotect crystals?
22.07.2014 16:18, Atul Kumar пишет:
Hi all,
I am trying to crystallize 92 Kda protein. I have got crystals in
Znso4, crystals are sensitive to radiation and died after few frames.
Unit cell parameters are a=94.53, b=11.49, c=39.69 (90, 102.3, 90) in
C2 space group (as suggested by mosflm). I tried to calculate mathews
coeff and it seems like only 10 kda protein can fit in the given unit
cell dimension with 44% solvent. Crystal appear in o/n and they have
sharp edges but after that I notice perturbation to crystal shape in
the drop. I was wondering whether I have crystals of degraded protein
or it is because of the poor latices?
I have also attached the image of diffraction pattern.
I would be happy to get useful suggestions.
thanks
regards
Atul
--
Eugene Osipov
Junior Research Scientist
Laboratory of Enzyme Engineering
A.N. Bach Institute of Biochemistry
Russian Academy of Sciences
Leninsky pr. 33, 119071 Moscow, Russia
e-mail: e.m.osi...@gmail.com
