Hi Frank -
How about a gel loading pipet tip as a substitute for the quartz
capillary? Suck the crystal into it, then try to get it to stick to the
wall. Flame seal the tip end, and use a glob of vacuum grease for the
other end (or cut off the skinny part with your crystal using a razor
blade).
That semi-transparent plastic FPLC tubing (Tefzel?) might work as a
substitute for the Mitegen capillary sleeve.
Your Xray absorption and background scattering will be really high from
all this plastic, but any port in a storm.
- Matt
On 7/7/14 12:32 PM, Frank von Delft wrote:
Hi all
Pretend you were stuck having to do RT data collection but without
access to either Mitegen MicroRT Capillaries or the more old-fashioned
quartz capillaries, to pop over the loop.
Anybody have suggestions of alternative ways of doing this? I do want
to use loops (I never learnt how to suck up crystals in capillaries).
I have access to a passably stocked biochemistry teaching lab, and
could at a pinch go rifle some more advanced research labs. (No, I'm
not at home ;)
Thanks!
phx
--
Matthew Franklin, Ph. D.
Senior Scientist
New York Structural Biology Center
89 Convent Avenue, New York, NY 10027
(212) 939-0660 ext. 9374
