Dear all, I would like to remove two phospholipids bound to (actually into) a cytoplasmic protein. The protein was expressed in E. coli and the crystal structure revealed the presence of two co-purified phospholipids (most probably DPPE).
A web search gave me three methods to remove bound lipids: - 1-butanol liquid–liquid extraction - Lipidex 1000, VI column at 37°C - HIC (aka Hydrophobic Interaction Chromatography) on a Phenyl HP column in presence of 1M ammonium sulphate All are described in Velkov 2008 (http://www.sciencedirect.com/science/article/pii/S1570023208002390). I assume these delipidation methods would also work for phospholipid; and I am more tempting by the last one, the milder one. I would appreciate any comment or practical advice. Best regards, Lionel
