Dear Shanti Looks like you’re looking down the symmetry axis - so this could simply be ‘noise’, or a superposition of two bound ligands on top of each other… what’s your cryo-protectant?
With regards, Tony. - - - - - - - - - - - - - - - - - - Dr Antony W Oliver Senior Research Fellow CR-UK DNA Repair Enzymes Group Genome Damage and Stability Centre Science Park Road University of Sussex Falmer, Brighton, BN1 9RQ - - - - - - - - - - - - - - - - - - email: antony.oli...@sussex.ac.uk<mailto:antony.oli...@sussex.ac.uk> tel (office): +44 (0)1273 678349 tel (lab): +44 (0)1273 677512 http://www.sussex.ac.uk/lifesci/oliverlab http://tinyurl.com/aw-oliver - - - - - - - - - - - - - - - - - - On 23 Jun 2014, at 13:17, Shanti Pal Gangwar <gangwar...@gmail.com<mailto:gangwar...@gmail.com>> wrote: Dear All I have solved a structure of my protein at 3.0 A. The crystallization condition is consisting of PEG400, NaCl, MgCl2 and Sodium citrate. The protein was purified in HEPES buffer. I can see an unidentified electron density blob in coot and I am not able to figure out what it could be? I have attached the snapshot of that blob with this mail. I request everyone to please help me in identification of this blob. Thanking you in advance. Shanti Pal ******************** Best regards Shanti Pal Gangwar, Ph.D School of Life Sciences Jawaharlal Nehru University New Delhi-110067 India Email:gangwar...@gmail.com<mailto:email%3agangwar...@gmail.com> <2.png><1.png>
