Dear ccp4 users, I get the following message from Phaser when I do a molecular replacement with two ensembles. One of the ensembles is placed but the second one is not placed, and then it says this:
Solutions with Z-scores greater than 13.0 (the threshold indicating a definite solution) were rejected for failing packing test #1 TFZ=16.8 PAK=487 #2 TFZ=17.4 PAK=440 #3 TFZ=17.0 PAK=312 #4 TFZ=16.7 PAK=294 #5 TFZ=16.8 PAK=227 #6 TFZ=16.6 PAK=284 #7 TFZ=16.2 PAK=219 #8 TFZ=16.3 PAK=287 #9 TFZ=16.1 PAK=186 #10 TFZ=16.6 PAK=277 #11 TFZ=16.7 PAK=204 #12 TFZ=16.8 PAK=404 #13 TFZ=15.9 PAK=271 #14 TFZ=15.8 PAK=194 #15 TFZ=14.9 PAK=229 #16 TFZ=16.4 PAK=368 #17 TFZ=17.3 PAK=194 #18 TFZ=15.2 PAK=240 #19 TFZ=16.1 PAK=325 #20 TFZ=15.3 PAK=455 #21 TFZ=16.5 PAK=298 #22 TFZ=16.6 PAK=290 #23 TFZ=15.2 PAK=259 #24 TFZ=16.2 PAK=194 #25 TFZ=16.1 PAK=314 #26 TFZ=16.3 PAK=194 #27 TFZ=16.3 PAK=387 #28 TFZ=15.6 PAK=193 #29 TFZ=15.7 PAK=219 #30 TFZ=15.6 PAK=474 #31 TFZ=15.1 PAK=194 #32 TFZ=15.4 PAK=339 #33 TFZ=15.5 PAK=210 #34 TFZ=15.2 PAK=300 #35 TFZ=15.6 PAK=186 #36 TFZ=16.2 PAK=216 #37 TFZ=14.9 PAK=182 #38 TFZ=15.7 PAK=279 #39 TFZ=15.5 PAK=285 #40 TFZ=15.0 PAK=374 #41 TFZ=15.4 PAK=404 #42 TFZ=15.3 PAK=185 #43 TFZ=15.6 PAK=227 #44 TFZ=14.8 PAK=364 #45 TFZ=15.7 PAK=193 #46 TFZ=14.5 PAK=448 #47 TFZ=14.6 PAK=219 #48 TFZ=15.5 PAK=210 #49 TFZ=15.7 PAK=189 #50 TFZ=15.0 PAK=193 #51 TFZ=15.0 PAK=281 #52 TFZ=15.2 PAK=202 And the list still continues for a bit. How should I think about this? I would assume that the clashes are too many only by seeing those numbers, but maybe there is something else to take into account? Thanks a lot in advance. Best wishes, Almudena -- Almudena Ponce-Salvatierra Macromolecular crystallography and Nucleic acid chemistry Max Planck Institute for Biophysical Chemistry Am Fassberg 11 37077 Göttingen Germany
