Dear All, Recently we collected some data of a MBP fusion protein, at around 4A resolution. The protein itself is about half of the MBP size. However when we tried to solve it with MR, it failed. We tried to use MBP alone, homology model of target protein alone, and MBP+model. It is very strange that MBP alone can not yield any reasonable solution at all, so does searching with MBP and model together. While searching with model alone could get some better results, but when fix it to search MBP, it failed. There are 1 molecule per ASU with solvent content 55%. The spacegroup should be right and we tried to search all possible alternatives in each run, we also tried to lower it down, but did not work either. When running Phenix.phaser, there is a warning at the beginning saying eLLG suggests placing of ensembles will be very difficult.
I wonder if anybody has encountered similar situation before. Any suggestions will be greatly appreciated! Regards, Niu
