Hi Everyone, In my 2.5-angstrom structure, there is negative Fo-Fc density surrounding a metal ion after refining in Phenix. From anomalous diffraction I am certain of the metal's identity and position in each monomer. Also, the ion is appropriately coordinated by nearby side chains. Should I be refining the occupancy of the ion in attempt to "flatten" the negative density? I am considering soaking the metal ion into crystals or cocrystallizing and collecting additional datasets.
Thanks for your help! Regards, Chris
