Hi, Another example is the Shiga-like toxin B-subunit pentamer (i.e. the cell-surface binding component of this A-B toxin), which binds 3 Gb3 trisaccharides per monomer:
http://pdbe.org/1bos http://www.ncbi.nlm.nih.gov/pubmed/9485303 Best wishes, Randy Read On 4 Mar 2014, at 10:41, Derek Logan <derek.lo...@biochemistry.lu.se> wrote: > Dear Wei, > > The enzyme ribonucleotide reductase can, depending on organism and class, > bind ATP as a substrate in the active site (c), as an allosteric regulator of > substrate specificity at another site (s) and as an overall activity > regulator at a third site (a)! It can also bind dATP at the second and third > sites, but not as a substrate. It cannot bind to sites (c) and (s) at the > same time although it could potentially bind to sites (s) and (a) > simultaneously. Here is a review that you might find useful: > > http://www.ncbi.nlm.nih.gov/pubmed/22050358 > > Best wishes > Derek > > On 4 Mar 2014, at 04:48, Wei Shi <wei.shi...@gmail.com> wrote: > >> Dear all, >> Does anyone happen to know examples of 2 ligands bind to a single protein / >> each monomer protein in 2 different ligand binding pockets? >> I know the following example: >> (1). phosphofructokinase, which binds ATP as both a ligand and a feedback >> inhibitor in different sites >> (2). 2 cAMP bound to each E. coli CAP monomer in the crystal structure. >> >> Does any of you know other examples? Thank you so much! >> >> Best, >> Wei ------ Randy J. Read Department of Haematology, University of Cambridge Cambridge Institute for Medical Research Tel: + 44 1223 336500 Wellcome Trust/MRC Building Fax: + 44 1223 336827 Hills Road E-mail: rj...@cam.ac.uk Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
