How about a short swish in well solution + 25-30% glucose? Doesn't take
long to cryoprotect, just a quick sufrace coat. Sodium malonate? We just
froze some really fragile crystals from 1.8 M sodium formate in 3 M
sodium malonate and they held up really well. (Still didn't improve
their diffraction, though--but at least they did not crack or disintegrate.)
_______________________________________
Roger S. Rowlett
Gordon & Dorothy Kline Professor
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: rrowl...@colgate.edu
On 2/18/2014 1:08 PM, Katherine Sippel wrote:
Hi all,
I'm looking for a cryo condition for high NaCl (3+ M) crystallization
condition. I would do it the proper way, but our beam/cryostream is down.
I've tried a bunch of things at the moment. Ethylene glycol and PEG
400 nuke the crystals immediately even at low concentrations.
Prolonged exposure to glycerol and sucrose starts to break them down
so I'm thinking that the diffraction will probably suffer. I can't
find any reports of NaCl's viability as a cryosalt. I've got
Paratone/Paraffin oil/Mitegen's LV cryo oil on tap but I was hoping to
not put all my eggs in one basket.
I tried the ISRDB database through archive.com <http://archive.com>
without any luck (no search function). I've gone to the PDB searching
for similar crystallization conditions and looked up the papers for
their cryos, but they are all glycerol. Google gives me the same.
I thought I'd see if anyone on the bb has an anecdotal "this worked
for us" story. I would love to hear it.
Thank you for your time,
Katherine
--
"Nil illegitimo carborundum"/- /Didactylos